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Signal enhancement by extravascular water protons, or SEEP, is a contrast mechanism for functional magnetic resonance imaging (fMRI), which is an alternative to the more commonly employed BOLD (blood-oxygen-level dependent) contrast. This mechanism for image contrast changes corresponding to changes in neuronal activity was first proposed by Dr. Patrick Stroman in 2001 (1, 2). SEEP contrast is based on changes in tissue water content which arise from the increased production of extracellular fluid (3, 4) and swelling of neurons and glial cells at sites of neuronal activity (5, 6). Because the dominant sources of MRI signal in biological tissues are water and lipids, an increase in tissue water content is reflected by a local increase in MR signal intensity. A correspondence between BOLD and SEEP signal changes, and sites of activity, has been observed in the brain and appears to arise from the common dependence on changes in local blood flow to cause a change in blood oxygenation or to produce extracellular fluid (7, 8). The advantage of SEEP contrast is that it can be detected with MR imaging methods which are relatively insensitive to magnetic susceptibility differences between air, tissues, blood, and bone. Such susceptibility differences can give rise to spatial image distortions and areas of low signal, and magnetic susceptibility changes in blood give rise to the BOLD contrast for fMRI. The primary application of SEEP to date has been fMRI of the spinal cord (spinal fMRI) because the bone/tissue interfaces around the spinal cord cause poor image quality with conventional fMRI methods. The disadvantages of SEEP compared to BOLD contrast are that it reveals more localized areas of activity, and in the brain the signal intensity changes are typically lower, and it can therefore be more difficult to detect (7-10). It is also controversial because it is not universally agreed to exist as a contrast mechanism for fMRI. However, more recent studies have demonstrated changes in MRI signal corresponding with changes in neuronal activity in rat cortical tissue slices, in the absence of blood flow or changes in oxygenation, and neuronal activity and cellular swelling were corroborated by light-transmittance microscopy. This demonstrated SEEP contrast in the absence of confounding factors which can occur in-vivo, such as physiological motion and the possibility of concurrent BOLD contrast. == References == ---- # Stroman PW, Krause V, Malisza KL, Frankenstein UN, Tomanek B. Characterization of contrast changes in functional MRI of the human spinal cord at 1.5 T. Magn Reson Imaging 2001;19(6):833-838. # Stroman PW, Krause V, Frankenstein UN, Malisza KL, Tomanek B. Spin-echo versus gradient-echo fMRI with short echo times. Magn Reson Imaging 2001;19(6):827-831. # # Fujita H, Meyer E, Reutens DC, Kuwabara H, Evans AC, Gjedde A. Cerebral () water clearance in humans determined by positron emission tomography: II. Vascular responses to vibrotactile stimulation. J Cereb Blood Flow Metab 1997;17(1):73-79. # Andrew RD, MacVicar BA. Imaging cell volume changes and neuronal excitation in the hippocampal slice. Neuroscience 1994;62(2):371-383. # Andrew RD, Jarvis CR, Obeidat AS. Potential sources of intrinsic optical signals imaged in live brain slices. Methods 1999;18(2):185-96, 179. # Stroman PW, Tomanek B, Krause V, Frankenstein UN, Malisza KL. Functional magnetic resonance imaging of the human brain based on signal enhancement by extravascular protons (SEEP fMRI). Magn Reson Med 2003;49(3):433-439. # Stroman PW, Kornelsen J, Lawrence J, Malisza KL. Functional magnetic resonance imaging based on SEEP contrast: response function and anatomical specificity. Magn Reson Imaging 2005;23(8):843-850. # Stroman PW, Krause V, Malisza KL, Frankenstein UN, Tomanek B. Extravascular proton-density changes as a non-BOLD component of contrast in fMRI of the human spinal cord. Magn Reson Med 2002;48(1):122-127. # Stroman PW, Malisza KL, Onu M. Functional magnetic resonance imaging at 0.2 Tesla. NeuroImage 2003;20(2):1210-1214. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「signal enhancement by extravascular water protons」の詳細全文を読む スポンサード リンク
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